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Insect cell protein expression
Protein expression technology can be applied to gene regulation analysis, protein structure and function analysis, protein-protein interaction analysis, etc.For specific applications, the use of the correct protein expression system is an important factor in the success of the experiment.In the selection of protein expression system, the most important factors to be considered are functionality, solubility, speed and yield.Sanyangkaipeptide Biotechnology Co., Ltd. has successfully constructed a complete platform for protein expression technology, including prokaryotic protein expression and purification, yeast protein expression and purification, insect cell protein expression and purification, mammalian cell protein expression and purification.
According to customers' needs, we provide one-stop services from gene synthesis, vector construction, gene expression, protein purification, detection, etc.
Sanyangkaipeptide Biotechnology Co., Ltd. has successfully constructed a complete platform for protein expression technology, including prokaryotic protein expression and purification, yeast protein expression and purification, insect cell protein expression and purification, mammalian cell protein expression and purification.According to customers' needs, we provide one-stop services from gene synthesis, vector construction, gene expression, protein purification, detection, etc.In order to ensure the quality of protein purification, sanyangkaipeptide biology has established a strict product quality control system, a complete set of customer service process and confidentiality system to ensure to provide customers with high-quality products and mature services.Our protein team has more experience in optimizing gene codon, design and construction of expression vector, selection of fusion object and label, selection of specific protein purification system, optimization of protein production process, etc.Can meet the personalized needs of customers.
Overview of baculovirus expression system in insects：
Baculovirus expression vector system (BEVS) is a eukaryotic expression system, which can fold and modify proteins after protein translation like mammalian cells. It is suitable to express some proteins that may have negative effects on mammalian host cells, such as kinases and toxic proteins.It is widely used in scientific research and industrial production.
Our technicians have rich experience in the expression of baculovirus recombinant proteins, especially for long-sequence proteins, active kinases and membrane proteins.Our technicians will optimize the output of baculovirus expression system in insects according to the specific properties of the protein to improve the expression of the protein.
Advantages of baculovirus expression system in insects：
1、Compared with other commonly used recombinant protein expression systems, baculovirus expression system has the advantages of high level of foreign gene expression, easy expression of Heteropoly protein, safety and so on. The expressed recombinant protein usually has the correct post-translational modification and biological activity, such as the formation of disulfide bond, phosphorylation, glycosylation and so on.
2、Insect cells grow in suspension and are easy to be enlarged for large-scale expression of recombinant proteins.
3、It is easy to express Heteropoly protein, which can be achieved by infecting insect cells with multiple recombinant viruses or by infecting insect cells with a virus containing multiple expression frames.
4、Safety.Baculoviruses do not infect vertebrates.
Service items and processes：
Trigoats provides one-stop protein expression technology services such as construction of baculovirus expression vector, expression detection, expression from large-scale cell culture and protein purification.
1、The target protein was cloned into baculovirus transfer vector;
2、The recombinant plasmid was prepared;
3、Insect cells were transfected to prepare virus;
4、Determination of virus titer;
5、The expression of recombinant protein was verified and the condition was optimized;
6、Small scale protein expression and purification (500 ml Culture);
7、Large scale protein expression and purification (optional, suitable for biomedical research enterprises).
Related technical services
Technical service for preparation of insect baculovirus
Protein expression optimization technology service
1、5-10ml recombinant baculovirus provided by the customer;
2、High titer virus was obtained by infecting insect cells;
3、Expression condition optimization.
Optimization factors include
Different insect cell lines;
Different expression time;
Different infection complex (MOI);
Trigoats provides one-stop protein expression technology services such as construction of baculovirus expression vector, expression detection, expression from large-scale cell culture and protein purification.
1、The target protein was cloned into baculovirus transfer vector;
2、The recombinant plasmid was prepared;
3、Insect cells were transfected to prepare virus;
4、Determination of virus titer;
5、The expression of recombinant protein was verified and the condition was optimized;
6、Small scale protein expression and purification (500 ml Culture);
7、Large scale protein expression and purification (optional, suitable for biomedical research enterprises).
Related technical services：
Technical service for preparation of insect baculovirus
Protein expression optimization technology service
1、5-10ml recombinant baculovirus provided by the customer;
2、High titer virus was obtained by infecting insect cells;
3、Expression condition optimization.
Optimization factors include:
Different insect cell lines;
Different expression time;
Different infection complex (MOI);
Virus titer determination service;
The titer was measured by plaque test.
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