Immune detection services

Immunoassay technology service

Immunoassay is based on the principle of antigen and antibody reaction, using known antigen to detect unknown antibody or using known antibody to detect unknown antigen. It can detect a specific protein qualitatively, localize and quantitatively.

Trigoats has a mature domestic immunoassay system, a large number of high-quality protein and antibody products support, advanced equipment and professional technical personnel, and can provide customers with a variety of immunoassay technology services: enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), immunoprecipitation (IP) and immunohistochemistry (IHC), etc.

一、Enzyme linked immunosorbent assay (ELISA)

ELISA is the most widely used technology in enzyme immunoassay.The basic method is to adsorb the known antigen or antibody on the surface of solid-phase carrier (polystyrene micro reaction plate), make the enzyme labeled antigen antibody react on the surface of solid-phase, and wash the free components in the liquid phase with washing method.The commonly used ELISA methods are double antibody sandwich method and indirect method. The former is used to detect macromolecular antigen, and the latter is used to detect specific antibody.

二、Western blot (WB)

WB is a method of transferring protein to membrane and then detecting it with antibody.For the known expressed protein, the corresponding antibody can be used as a primary antibody for detection. For the expression product of the new gene, the antibody of the fusion part can be used for detection.WB uses polyacrylamide gel electrophoresis, the detected protein is the "probe" is the antibody, and the "chromogenic" uses the marked two resistance.
The protein samples separated by page were transferred to the solid-phase carrier (such as nitrocellulose film). The solid-phase carrier adsorbed the protein in the form of non covalent bond, and kept the type and biological activity of the peptides separated by electrophoresis unchanged.The protein or polypeptide on the solid-phase carrier is used as the antigen, which reacts with the corresponding antibody, and then reacts with the second antibody labeled by enzyme or isotope. The protein components expressed by the specific target gene separated by electrophoresis are detected by substrate color or autoradiography.This technique is also widely used to detect protein expression.

三、Immunoprecipitation (IP)

Immunoprecipitation (IP) is a method to purify proteins, incubating antibodies and cellular extracts of target proteins, so that antibodies and proteins are bound to the solution. Then, the agarose gel coupled with protein A/G is used to extract the antibody antigen complex from the sample. This physical method can separate the required protein from the sample, and then the sample can be used. It was separated by SDS-PAGE and analyzed by Western blot.

四、Immunohistochemistry (IHC)

Immunohistochemical (IHC) staining is a method that uses the specific reaction of antigen and antibody to locate or qualitatively analyze the antigen substance in cells or tissues.Its operation is relatively simple, with high sensitivity and specificity.It has high application value in clinical diagnosis.


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